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293gp packaging cell line  (TaKaRa)


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    Structured Review

    TaKaRa 293gp packaging cell line
    293gp Packaging Cell Line, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/293gp+packaging+cell+line/Pantropic+Retroviral+Expression+System/pm41545542-254-11-15
    Average 95 stars, based on 105 article reviews
    293gp packaging cell line - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Apoptosis-associated speck-like protein containing a caspase recruitment domain is a regulator of procaspase-1 activation.
    Article Snippet: Transfection of HEK293 cells was accomplished using Superfect (Qiagen, Alameda, CA), whereas COS-7 and HeLa cells were transfected with LipoFectamine Plus (Life Technologies), holding total DNA content constant. .. The 293GP packaging cell line (Clontech) was stably transfected with either pMSCV-Myc-ASC or pMSCV-Neo, and stable clones were selected by culturing in 200 ng/ml hygromycin (Calbiochem, La Jolla, CA). .. These cells were then transiently transfected with pVSV-G (Clontech Laboratories) in 60-mm dishes and vesicular stomatitis virus (VSV)-G-pseudotyped retrovirus-containing culture supernatants (3 ml/dish) were collected 48 h later, clarified by filtration, and used to infect 107 THP-1 cells for 90 min at 2500 rpm at 32°C followed by 5 h at 32°C.

    Transfection:

    Article Title: Apoptosis-associated speck-like protein containing a caspase recruitment domain is a regulator of procaspase-1 activation.
    Article Snippet: Transfection of HEK293 cells was accomplished using Superfect (Qiagen, Alameda, CA), whereas COS-7 and HeLa cells were transfected with LipoFectamine Plus (Life Technologies), holding total DNA content constant. .. The 293GP packaging cell line (Clontech) was stably transfected with either pMSCV-Myc-ASC or pMSCV-Neo, and stable clones were selected by culturing in 200 ng/ml hygromycin (Calbiochem, La Jolla, CA). .. These cells were then transiently transfected with pVSV-G (Clontech Laboratories) in 60-mm dishes and vesicular stomatitis virus (VSV)-G-pseudotyped retrovirus-containing culture supernatants (3 ml/dish) were collected 48 h later, clarified by filtration, and used to infect 107 THP-1 cells for 90 min at 2500 rpm at 32°C followed by 5 h at 32°C.

    Clone Assay:

    Article Title: Apoptosis-associated speck-like protein containing a caspase recruitment domain is a regulator of procaspase-1 activation.
    Article Snippet: Transfection of HEK293 cells was accomplished using Superfect (Qiagen, Alameda, CA), whereas COS-7 and HeLa cells were transfected with LipoFectamine Plus (Life Technologies), holding total DNA content constant. .. The 293GP packaging cell line (Clontech) was stably transfected with either pMSCV-Myc-ASC or pMSCV-Neo, and stable clones were selected by culturing in 200 ng/ml hygromycin (Calbiochem, La Jolla, CA). .. These cells were then transiently transfected with pVSV-G (Clontech Laboratories) in 60-mm dishes and vesicular stomatitis virus (VSV)-G-pseudotyped retrovirus-containing culture supernatants (3 ml/dish) were collected 48 h later, clarified by filtration, and used to infect 107 THP-1 cells for 90 min at 2500 rpm at 32°C followed by 5 h at 32°C.

    Retroviral:

    Article Title: BACH2 dosage establishes the hierarchy of stemness and fine-tunes antitumor immunity in CAR T cells.
    Article Snippet: .. For transducing human T cells, retroviral supernatants were generated using the 293GP packaging cell line (Takara, 631512). .. Briefly, 293GP cells were co-transfected with retroviral and RD114 envelope plasmids using TransIT-293 Transfection Reagent (Mirus Bio) in Opti-MEM (Thermo Fisher Scientific).

    Article Title: BACH2 dosage establishes the hierarchy of stemness and fine-tunes antitumor immunity in CAR T cells
    Article Snippet: .. For transducing human T cells, retroviral supernatants were generated using the 293GP packaging cell line (Takara, 631512). .. Briefly, 293GP cells were co-transfected with retroviral and RD114 envelope plasmids using TransIT-293 Transfection Reagent (Mirus Bio) in Opti-MEM (Thermo Fisher Scientific).

    Generated:

    Article Title: BACH2 dosage establishes the hierarchy of stemness and fine-tunes antitumor immunity in CAR T cells.
    Article Snippet: .. For transducing human T cells, retroviral supernatants were generated using the 293GP packaging cell line (Takara, 631512). .. Briefly, 293GP cells were co-transfected with retroviral and RD114 envelope plasmids using TransIT-293 Transfection Reagent (Mirus Bio) in Opti-MEM (Thermo Fisher Scientific).

    Article Title: BACH2 dosage establishes the hierarchy of stemness and fine-tunes antitumor immunity in CAR T cells
    Article Snippet: .. For transducing human T cells, retroviral supernatants were generated using the 293GP packaging cell line (Takara, 631512). .. Briefly, 293GP cells were co-transfected with retroviral and RD114 envelope plasmids using TransIT-293 Transfection Reagent (Mirus Bio) in Opti-MEM (Thermo Fisher Scientific).



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